Overview | Culturing | DNA extraction | DNA quantification | Library preparation | Data analysis | Laboratory Manual | Costs
This document accompanies the recently published paper and is an indepth handbook covering all aspects of the pipeline. This handbook is designed to cover a pragmatic whole genome sequencing (WGS) system for Mycobacterium tuberculosis for laboratories who want to utilise long read technology or with little/no access to larger scale Illumina sequencing platforms. It is designed to identify lineages (which can be of both clinical and epidemiological use), and antimicrobial resistances (e.g. for hard-to-treat cases, or to ensure early treatment is appropriate).
*NEW* The protocol booklet for our tuberculosis ONT pipeline is now available here!